UTR's are untranslated regions of a mRNA. It can be downstream or upstream of the actual mRNA. UTR's are shown to be able to regulate where genes are expressed.
Experiment from: Evans, T, Translational control of maternal glp-1 mRNA...
Expression of glp-1 mRNA is monitored in a four-celled stage of C. Elegans. Glp-1 mRNA is in all 4 cells, but only expressed in 2. A LacZ gene is put in place of glp-1, but the original UTR is left where it is. It shows then that the lacZ gene is expressed in the 2 cells where the glp-1 gene was expressed. This implies that the UTR:glp-1 regulates the location-specific translation.
Then, the UTR in that engineered lacZ:UTR-glp-1 segment is altered. As a result, the specific two cell expression of lacZ is obstructed. As the UTR is changed in structure, it shows that the UTR of glp-1 is responsible for regulation.
Conclusion: certain regions in mRNA can regulate expression, and the instruction can be found in the untranslated region (UTR) of the mRNA. Obstructing the UTR shows differences in expression, proving that the UTR plays a role in expression.
p33 F2.12
No comments:
Post a Comment